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The intra- and inter-day

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precision and accuracy of the quality control (QC) samples were. Analysis was performed in the

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positive ion mode using atmospheric

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pressure chemical ionization birth control oral contraceptives (APCI). The standard curve range was 2.0-1000 ng/mL for

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each Fluoxetine ( Prozac ) enantiomer.

Where possible, sample transfer and LLE steps were automated using a Tomtec Quadra 96 workstation.

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A high-throughput sample preparation

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procedure using liquid-liquid extraction (LLE) in a 96-well plate format

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in conjunction

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with liquid chromatography/tandem mass spectrometry (LC/MS/MS) was developed and validated for quantification of Fluoxetine ( Prozac ) enantiomers in human

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plasma. The organic extract was

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evaporated to dryness and reconstituted in methanol. After addition of internal standard and ammonium hydroxide,

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samples were extracted

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with daniela acetate.

Enantiomeric separation and quantification of Fluoxetine ( Prozac ) (Prozac) in human plasma by liquid chromatography/tandem mass spectrometry using liquid-liquid extraction in 96-well plate format.Fluoxetine ( Prozac ) (Prozac) is currently one of the widely prescribed selective serotonin reuptake inhibitors (SSRIs) for the treatment of depression. Adequate separation of Fluoxetine ( Prozac ) enantiomeric pairs (resolution of 1.17) was achieved on a vancomycin column eluted with methanol containing 0.075% (by weight) ammonium trifluoroacetate. A triple quadrupole mass spectrometer, operated in the multiple reaction monitoring mode at m/z 310–>44 for Fluoxetine ( Prozac ) enantiomeric pairs and m/z 287–>241 drug store news editor

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for oxazepam (internal standard), was used.

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